Outcomes of combined mitochondria and mesenchymal stem cells-derived exosome therapy in rat acute respiratory distress syndrome and sepsis

Figure 8 Protein expressions of mitochondrial electron transport chain complexes and mitofusin 2 by day 5 after acute respiratory distress syndrome induction. A: Protein expression of complex I, control vs other groups with different letters, ^aP < 0.0001, ^bP < 0.001, ^cP < 0.01; B: Protein expression of complex II, control vs other groups with different letters, ^aP < 0.0001, ^bP < 0.001, ^cP < 0.01; C: Protein expression of complex III, control vs other groups with different letters, ^aP < 0.0001, ^bP < 0.001, ^cP < 0.01; D: Protein expression of complex IV, control vs other groups with different letters, ^aP < 0.0001, ^bP < 0.001, ^cP < 0.01; E: Protein expression of complex V, control vs other groups with different letters, ^aP < 0.0001, ^bP < 0.01, ^cP < 0.001, ^dP < 0.05; F: Protein expression of mitofusin 2, control vs other groups with different letters, ^aP < 0.0001, ^bP < 0.01, ^cP < 0.05; G-K: Illustrating the microscopic finding (400 ×) of immunohistochemical stain for identification of 8-hydroxy-2’-deoxyguanosine (8-OHdG)+ cells (gray color). The scale bars in right lower corner represent 20 μm; L: Analytical result of number of 8-OHdG+ cells, control vs other groups with different letters, ^aP < 0.0001, ^bP < 0.005, ^cP < 0.01, ^dP < 0.001. All statistical analyses were performed by one-way ANOVA, followed by Bonferroni multiple comparison post hoc test (n = 6 for each group). CI: Complex I; CII: Complex II; CIII: Complex III; CIV: Complex IV; CV: Complex V; Mfn2: Mitofusin 2; 8-OHdG: 8-hydroxy-2’-deoxyguanosine; SC: Sham-operated control; ARDS: Acute respiratory distress syndrome; SS: Sepsis syndrome; mito^Ex: Exogenous mitochondria; EX^AD: Adipose-derived mesenchymal stem cells-derived exosomes.