Outcomes of combined mitochondria and mesenchymal stem cells-derived exosome therapy in rat acute respiratory distress syndrome and sepsis

Figure 5 Levels of inflammatory cells and albumin in bronchoalveolar lavage fluid and circulatory proinflammatory cytokine by day 5 after acute respiratory distress syndrome induction. A: Enzyme-linked immunosorbent assay analysis of tumor necrosis factor (TNF)-α level in bronchoalveolar lavage (BAL), control vs other groups with different letters, ^aP < 0.0001, ^bP < 0.01, ^cP < 0.05, ^dP < 0.001; B: Flow cytometric analysis of number of Ly6G+ cells, control vs other groups with different letters, ^aP < 0.0001, ^bP < 0.01, ^cP < 0.05, ^dP < 0.001; C: Flow cytometric analysis of number of CD11^b/c+ cells, control vs other groups with different letters, ^aP < 0.0001, ^bP < 0.01, ^cP < 0.05, ^dP < 0.001; D: Flow cytometric analysis of number of myeloperoxidase+ cells, control vs other groups with different letters, ^aP < 0.0001, ^bP < 0.01, ^cP < 0.05, ^dP < 0.001; E: Relative mitochondrial DNA level, control vs other groups with different letters, ^aP < 0.01, ^bP < 0.05, ^cP < 0.001, ^dP < 0.0001; F: Circulatory level of TNF-α, control vs other groups with different letters, ^aP < 0.0001, ^bP < 0.05, ^cP < 0.01, ^dP < 0.001; G: Circulatory level of interleukin-6, control vs other groups with different letters, ^aP < 0.0001, ^bP < 0.05, ^cP < 0.01, ^dP < 0.001; H: The mortality rate by day 5 after acute respiratory distress syndrome induction, control vs^aP < 0.05, control vs^bP > 0.05; I: Analytical result of BAL albumin concentration, control vs other groups with different letters, ^aP < 0.0001, ^bP < 0.01, ^cP < 0.05, ^dP < 0.001. All statistical analyses were performed by one-way ANOVA, followed by Bonferroni multiple comparison post hoc test (n = 6 for each group). IL: Interleukin; TNF: Tumor necrosis factor; MPO: Myeloperoxidase; SC: Sham-operated control; ARDS: Acute respiratory distress syndrome; SS: Sepsis syndrome; mito^Ex: Exogenous mitochondria; EX^AD: Adipose-derived mesenchymal stem cells-derived exosomes; BAL: Bronchoalveolar lavage.