Outcomes of combined mitochondria and mesenchymal stem cells-derived exosome therapy in rat acute respiratory distress syndrome and sepsis

Figure 1 Qualitative exosome biomarkers and oxygen consumption rate of mitochondria. A: Illustrating the protein expressions of CD63, TSG101 and β-catenin. The result showed that the protein expressions of these three fundamental biomarkers of exosome were notably progressively increased as the concentration of exosomes was stepwise increased from 1 μg, 2 μg, 10 μg, to 50 μg; B: Illustrating the oxygen consumption rate (OCR) curve; the result showed that the mitochondrial respiration reflected by the level of OCR was remarkably high, implicating that the isolated mitochondria had excellent functional integrity and efficacy. A: Untreated; B: Oligomycin; C: Carbonyl cyanide-p-trifluoromethoxyphenylhydrazone; D: Actinomycin A/rotenone. OCR: Oxygen consumption rate; FCCP: Carbonyl cyanide-p-trifluoromethoxyphenylhydrazone.