RPLP0/TBP are the most stable reference genes for human dental pulp stem cells under osteogenic differentiation

Figure 4 Effect of the reference gene (glyceraldehyde-3-phosphate dehydrogenase or TATA-binding protein/ribosomal protein, large, P0) on the gene expression of RUNX2. A: Normalization data of all samples (from the three donors) under clonogenic or osteogenic media by glyceraldehyde-3-phosphate dehydrogenase (GAPDH); B: Normalization data of all samples (from the three donors) under clonogenic or osteogenic media by TATA-binding protein (TBP)/ribosomal protein, large, P0 (RPLP0); C: Normalization data of only donor#1 under clonogenic or osteogenic media by GAPDH; D: Normalization data of only donor #1 under clonogenic or osteogenic media by TBP/RPLP0; E: Normalization data of only donor #2 under clonogenic or osteogenic media by GAPDH; F: Normalization data of only donor #2 under clonogenic or osteogenic media by TBP/RPLP0; G: Normalization data of only donor #2 under clonogenic or osteogenic media by GAPDH; H: Normalization data of only donor #3 under clonogenic or osteogenic media by TBP/RPLP0. RPLP0: Ribosomal protein, large, P0; TBP: TATA box binding protein; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase; RUNX2: Runt-related transcription factor 2.