RPLP0/TBP are the most stable reference genes for human dental pulp stem cells under osteogenic differentiation

doi:10.4252/wjsc.v16.i6.656

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World Journal of Stem Cells

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1948-0210

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World J Stem Cells. Jun 26, 2024; 16(6): 656-669
Published online Jun 26, 2024. doi: 10.4252/wjsc.v16.i6.656

Table 6 Gene stability from Subset 7 (only clonogenic medium from 1 to 21 d) and Subset 8 (only clonogenic medium from 21 to 35 d)

Subset	Rank	Delta Ct		NormFinder		geNorm		BestKeeper
Subset	Rank	Gene	Stability value	Gene	Stability value	Gene	M value	Gene	Std Dev	R	P value
7: 1 to 21 d (clo)	1	TBP	2.010	TBP	1.091	RPLP0/EFα1	0.661	ALAS1	0.690	0.379	0.051
	2	RPLP0	2.120	RPLP0	1.379	-	-	EEF1α1	0.820	0.144	0.473
	3	YWAHZ	2.150	YWAHZ	1.398	ALAS1	1.033	RPLP0	0.870	0.516	0.006
	4	ALAS1	2.150	ALAS1	1.404	TBP	1.430	TBP	1.600	0.778	0.001
	5	B2M	2.210	B2M	1.442	YWAHZ	1.617	GAPDH	1.630	0.722	0.001
	6	EFα1	2.370	GAPDH	1.615	B2M	1.718	YWAHZ	1.950	0.759	0.001
	7	GAPDH	2.370	EFα1	1.839	SDHA	1.890	B2M	1.980	0.762	0.001
	8	SDHA	2.610	SDHA	2.089	GAPDH	2.032	ACTB	2.180	0.789	0.001
	9	ACTB	2.630	ACTB	2.133	ACTB	2.212	SDHA	2.320	0.800	0.001
	10	TUB	2.920	TUB	2.546	TUB	2.354	TUB	2.660	0.815	0.001
8: 21 to 35 d (clo)	1	TBP	2.070	TBP	1.063	ACTB/TUB	0.766	ALAS1	0.670	0.480	0.044
	2	RPLP0	2.130	RPLP0	1.247	-	-	GAPDH	1.470	0.455	0.058
	3	TUB	2.200	TUB	1.378	RPLP0	1.417	RPLP0	1.680	0.841	0.001
	4	ACTB	2.210	ACTB	1.430	GAPDH	1.621	EEF1α1	1.780	0.752	0.001
	5	YWAHZ	2.280	YWAHZ	1.601	ALAS1	1.757	ACTB	1.780	0.801	0.001
	6	ALAS1	2.480	ALAS1	1.813	EEF1α1	1.932	TUB	1.850	0.799	0.001
	7	EFα1	2.580	EFα1	1.953	TBP	2.055	TBP	2.170	0.901	0.001
	8	GAPDH	2.680	B2M	2.231	YWAHZ	2.172	YWAHZ	2.520	0.862	0.001
	9	B2M	2.700	GAPDH	2.232	B2M	2.315	B2M	3.070	0.857	0.001
	10	SDHA	2.820	SDHA	2.388	SDHA	2.415	SDHA	3.240	0.884	0.001

ACTB: Actin beta; ALAS1: Aminolevulinic acid synthase 1; B2M: Beta-2-microglobulin; clo: Clonogenic EF1α: Eukaryotic translational elongation factor 1 alpha; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase; RPLP0: Ribosomal protein, large, P0; SDHA: Succinate dehydrogenase complex, subunit A, flavoprotein; Std Dev: Standard deviation; TBP: TATA-binding protein; TUB: Tubulin; YWHAZ: Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta.

Citation: Ferreira DB, Gasparoni LM, Bronzeri CF, Paiva KBS. RPLP0/TBP are the most stable reference genes for human dental pulp stem cells under osteogenic differentiation. World J Stem Cells 2024; 16(6): 656-669
URL: https://www.wjgnet.com/1948-0210/full/v16/i6/656.htm
DOI: https://dx.doi.org/10.4252/wjsc.v16.i6.656