RPLP0/TBP are the most stable reference genes for human dental pulp stem cells under osteogenic differentiation

doi:10.4252/wjsc.v16.i6.656

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World Journal of Stem Cells

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1948-0210

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Stem Cells. Jun 26, 2024; 16(6): 656-669
Published online Jun 26, 2024. doi: 10.4252/wjsc.v16.i6.656

Table 5 Gene stability from Subset 5 (only osteogenic medium from 1 to 21 d) and Subset 6 (only osteogenic medium from 21 to 35 d)

Subset	Rank	Delta Ct		NormFinder		geNorm		BestKeeper
Subset	Rank	Gene	Stability value	Gene	Stability value	Gene	M value	Gene	Std Dev	R	P value
5: 1 to 21 d (ost)	1	EFα1	2.200	EFα1	1.255	RPLP0/EFα1	0.463	ALAS1	0.810	0.440	0.022
	2	RPLP0	2.210	RPLP0	1.278	-	-	RPLP0	1.330	0.731	0.001
	3	TBP	2.280	TBP	1.325	ACTB	1.514	EFα1	1.330	0.755	0.001
	4	ALAS1	2.380	ALAS1	1.485	TUB	1.629	ACTB	1.540	0.555	0.003
	5	YWAHZ	2.450	YWAHZ	1.706	ALAS1	1.742	TBP	1.860	0.787	0.001
	6	ACTB	2.480	ACTB	1.757	TBP	1.978	TUB	1.880	0.620	0.001
	7	TUB	2.600	TUB	1.948	YWAHZ	2.115	YWAHZ	2.170	0.845	0.001
	8	SDHA	2.660	SDHA	2.043	SDHA	2.222	B2M	2.240	0.609	0.001
	9	B2M	2.950	B2M	2.369	B2M	2.345	SDHA	2.290	0.752	0.001
	10	GAPDH	3.460	GAPDH	3.031	GAPDH	2.568	GAPDH	2.310	0.647	0.001
6: 21 to 35 d (ost)	1	TBP	1.820	TBP	0.578	RPLP0/EFα1	0.495	ALAS1	0.560	0.257	0.304
	2	ACTB	1.900	ACTB	0.584	-	-	TBP	1.750	0.963	0.001
	3	RPLP0	2.050	RPLP0	1.096	ACTB	1.024	RPLP0	1.810	0.878	0.001
	4	EFα1	2.110	EFα1	1.224	TBP	1.134	EFα1	2.040	0.883	0.001
	5	SDHA	2.160	SDHA	1.368	SDHA	1.440	ACTB	2.070	0.963	0.001
	6	YWAHZ	2.300	TUB	1.551	YWAHZ	1.557	SDHA	2.250	0.843	0.001
	7	TUB	2.310	YWAHZ	1.648	TUB	1.655	TUB	2.400	0.856	0.001
	8	ALAS1	2.830	ALAS1	2.254	B2M	1.842	GAPDH	2.510	0.492	0.038
	9	B2M	2.840	B2M	2.401	ALAS1	2.035	YWAHZ	2.760	0.927	0.001
	10	GAPDH	4.040	GAPDH	3.776	GAPDH	2.435	B2M	2.800	0.810	0.001

ACTB: Actin beta; ALAS1: Aminolevulinic acid synthase 1; B2M: Beta-2-microglobulin; EF1α: Eukaryotic translational elongation factor 1 alpha; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase; ost: Osteogenic; RPLP0: Ribosomal protein, large, P0; SDHA: Succinate dehydrogenase complex, subunit A, flavoprotein; Std Dev: Standard deviation; TBP: TATA-binding protein; TUB: Tubulin; YWHAZ: Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta.

Citation: Ferreira DB, Gasparoni LM, Bronzeri CF, Paiva KBS. RPLP0/TBP are the most stable reference genes for human dental pulp stem cells under osteogenic differentiation. World J Stem Cells 2024; 16(6): 656-669
URL: https://www.wjgnet.com/1948-0210/full/v16/i6/656.htm
DOI: https://dx.doi.org/10.4252/wjsc.v16.i6.656