RPLP0/TBP are the most stable reference genes for human dental pulp stem cells under osteogenic differentiation

doi:10.4252/wjsc.v16.i6.656

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World Journal of Stem Cells

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1948-0210

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World J Stem Cells. Jun 26, 2024; 16(6): 656-669
Published online Jun 26, 2024. doi: 10.4252/wjsc.v16.i6.656

Table 4 Gene stability from Subset 3 (clonogenic and osteogenic media from 1 to 21 d) and Subset 4 (clonogenic and osteogenic media from 21 to 35 d)

Subset	Rank	Delta Ct		Normfinder		geNorm		BestKeeper
Subset	Rank	Gene	Stability value	Gene	Stability value	Gene	M value	Gene	Std Dev	R	P value
3: 1 to 21 d (clo + ost)	1	TBP	2.160	TBP	1.202	RPLP0/EFα1	0.594	ALAS1	0.750	0.433	0.001
	2	RPLP0	2.230	RPLP0	1.419	-	-	RPLP0	1.100	0.614	0.001
	3	YWAHZ	2.320	ALAS1	1.492	ALAS1	1.207	EFα1	1.100	0.535	0.001
	4	EFα1	2.320	YWAHZ	1.550	TBP	1.639	TBP	1.770	0.801	0.001
	5	ALAS1	2.330	EEF1α1	1.591	YWAHZ	1.814	ACTB	1.940	0.703	0.001
	6	ACTB	2.580	ACTB	1.938	SDHA	1.989	GAPDH	2.010	0.683	0.001
	7	SDHA	2.640	B2M	1.979	B2M	2.104	YWAHZ	2.080	0.819	0.001
	8	B2M	2.640	SDHA	2.048	ACTB	2.275	B2M	2.210	0.709	0.001
	9	TUB	2.790	TUB	2.252	TUB	2.383	SDHA	2.290	0.785	0.001
	10	GAPDH	2.970	GAPDH	2.411	GAPDH	2.499	TUB	2.350	0.738	0.001
4: 21 to 35 d (clo + ost)	1	TBP	1.980	TBP	0.749	RPLP0/EFα1	1.039	ALAS1	0.630	0.345	0.039
	2	ACTB	2.090	ACTB	1.075	-	-	RPLP0	1.740	0.860	0.001
	3	RPLP0	2.120	RPLP0	1.155	ACTB	1.427	EFα1	1.890	0.815	0.001
	4	TUB	2.280	TUB	1.447	TBP	1.636	ACTB	1.920	0.893	0.001
	5	YWAHZ	2.300	YWAHZ	1.604	TUB	1.738	TBP	1.940	0.915	0.001
	6	EFα1	2.360	EEF1α1	1.605	YWAHZ	1.866	GAPDH	1.970	0.456	0.005
	7	SDHA	2.540	SDHA	1.979	SDHA	1.980	TUB	2.120	0.829	0.001
	8	ALAS1	2.670	ALAS1	2.044	B2M	2.087	YWAHZ	2.620	0.899	0.001
	9	B2M	2.760	B2M	2.283	ALAS1	2.210	SDHA	2.810	0.838	0.001
	10	GAPDH	3.420	GAPDH	3.075	GAPDH	2.452	B2M	2.930	0.830	0.001

ACTB: Actin beta; ALAS1: Aminolevulinic acid synthase 1; B2M: Beta-2-microglobulin; clo: Clonogenic; EF1α: Eukaryotic translational elongation factor 1 alpha; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase; ost: Osteogenic; RPLP0: Ribosomal protein, large, P0; SDHA: Succinate dehydrogenase complex, subunit A, flavoprotein; Std Dev: Standard deviation; TBP: TATA-binding protein; TUB: Tubulin; YWHAZ: Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta.

Citation: Ferreira DB, Gasparoni LM, Bronzeri CF, Paiva KBS. RPLP0/TBP are the most stable reference genes for human dental pulp stem cells under osteogenic differentiation. World J Stem Cells 2024; 16(6): 656-669
URL: https://www.wjgnet.com/1948-0210/full/v16/i6/656.htm
DOI: https://dx.doi.org/10.4252/wjsc.v16.i6.656