RPLP0/TBP are the most stable reference genes for human dental pulp stem cells under osteogenic differentiation

doi:10.4252/wjsc.v16.i6.656

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World Journal of Stem Cells

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1948-0210

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World J Stem Cells. Jun 26, 2024; 16(6): 656-669
Published online Jun 26, 2024. doi: 10.4252/wjsc.v16.i6.656

Table 3 Gene stability from Subset 1 (clonogenic medium under 35 d) and Subset 2 (osteogenic medium under 35 d)

Subset	Rank	Delta Ct		NormFinder		geNorm		BestKeeper
Subset	Rank	Gene	Stability value	Gene	Stability value	Gene	M value	Gene	Std Dev	R	P value
1: 1 to 35 d (clo)	1	TBP	2.040	TBP	1.024	TBP/YWAHZ	0.996	ALAS1	0.660	0.326	0.043
	2	RPLP0	2.220	RPLP0	1.423	-	-	RPLP0	1.200	0.700	0.001
	3	YWAHZ	2.220	YWAHZ	1.466	B2M	1.319	EFα1	1.250	0.537	0.001
	4	B2M	2.430	B2M	1.790	SDHA	1.449	GAPDH	1.660	0.647	0.001
	5	ACTB	2.460	ACTB	1.818	RPLP0	1.880	TBP	1.900	0.866	0.001
	6	ALAS1	2.480	ALAS1	1.821	EFα1	2.068	ACTB	2.140	0.799	0.001
	7	GAPDH	2.500	GAPDH	1.824	ALAS1	2.159	YWAHZ	2.240	0.850	0.001
	8	EFα1	2.560	EFα1	1.989	GAPDH	2.272	TUB	2.510	0.800	0.001
	9	SDHA	2.620	SDHA	2.099	ACTB	2.356	B2M	2.560	0.855	0.001
	10	TUB	2.680	TUB	2.161	TUB	2.421	SDHA	2.710	0.875	0.001
2: 1 to 35 d (ost)	1	TBP	2.140	TBP	1.087	RPLP0/EFα1	0.515	ALAS1	0.740	0.324	0.044
	2	RPLP0	2.170	RPLP0	1.276	-	-	RPLP0	1.460	0.786	0.001
	3	EFα1	2.180	EFα1	1.291	TBP	1.450	EFα1	1.570	0.812	0.001
	4	ACTB	2.320	ACTB	1.467	YWAHZ	1.777	ACTB	1.720	0.767	0.001
	5	YWAHZ	2.450	YWAHZ	1.758	SDHA	1.860	TBP	1.850	0.846	0.001
	6	SDHA	2.480	SDHA	1.789	ACTB	2.010	GAPDH	1.920	0.519	0.001
	7	TUB	2.570	ALAS1	1.853	TUB	2.108	TUB	2.190	0.744	0.001
	8	ALAS1	2.570	TUB	1.882	ALAS1	2.184	SDHA	2.460	0.818	0.001
	9	B2M	2.970	B2M	2.456	B2M	2.323	YWAHZ	2.530	0.901	0.001
	10	GAPDH	3.270	GAPDH	2.833	GAPDH	2.512	B2M	2.640	0.739	0.001

ACTB: Actin beta; ALAS1: Aminolevulinic acid synthase 1; B2M: Beta-2-microglobulin; clo: Clonogenic; EF1α: Eukaryotic translational elongation factor 1 alpha; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase; ost: Osteogenic; RPLP0: Ribosomal protein, large, P0; SDHA: Succinate dehydrogenase complex, subunit A, flavoprotein; Std Dev: Standard deviation; TBP: TATA-binding protein; TUB: Tubulin; YWHAZ: Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta.

Citation: Ferreira DB, Gasparoni LM, Bronzeri CF, Paiva KBS. RPLP0/TBP are the most stable reference genes for human dental pulp stem cells under osteogenic differentiation. World J Stem Cells 2024; 16(6): 656-669
URL: https://www.wjgnet.com/1948-0210/full/v16/i6/656.htm
DOI: https://dx.doi.org/10.4252/wjsc.v16.i6.656