RPLP0/TBP are the most stable reference genes for human dental pulp stem cells under osteogenic differentiation

doi:10.4252/wjsc.v16.i6.656

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World Journal of Stem Cells

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1948-0210

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Stem Cells. Jun 26, 2024; 16(6): 656-669
Published online Jun 26, 2024. doi: 10.4252/wjsc.v16.i6.656

Table 2 Gene stability of overall analysis from clonogenic and osteogenic samples

Main group	Rank	Delta Ct		NormFinder		geNorm		BestKeeper
Main group	Rank	Gene	Stability value	Gene	Stability value	Gene	M value	Gene	Std Dev	R	P value
1 to 35 d (clo + ost)	1	TBP	2.13	TBP	1.078	RPLP0/EFα1	0.889	ALAS1	0.70	0.335	0.003
	2	RPLP0	2.27	RPLP0	1.451	-	-	RPLP0	1.33	0.726	0.001
	3	YWAHZ	2.37	YWAHZ	1.62	TBP	1.712	EFα1	1.41	0.668	0.001
	4	EFα1	2.43	ACTB	1.659	YWAHZ	1.923	GAPDH	1.78	0.571	0.001
	5	ACTB	2.43	EFα1	1.707	SDHA	2.037	TBP	1.87	0.857	0.001
	6	ALAS1	2.55	ALAS1	1.84	ALAS1	2.152	ACTB	2.00	0.785	0.001
	7	SDHA	2.57	SDHA	1.948	ACTB	2.261	YWAHZ	2.40	0.876
	8	TUB	2.68	TUB	2.06	B2M	2.337	TUB	2.41	0.777
	9	B2M	2.72	B2M	2.122	TUB	2.402	SDHA	2.59	0.847
	10	GAPDH	2.93	GAPDH	2.385	GAPDH	2.508	B2M	2.63	0.799	0.001

ACTB: Actin beta; ALAS1: Aminolevulinic acid synthase 1; B2M: Beta-2-microglobulin; clo: Clonogenic; EF1α: Eukaryotic translational elongation factor 1 alpha; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase; ost: Osteogenic; RPLP0: Ribosomal protein, large, P0; SDHA: Succinate dehydrogenase complex, subunit A, flavoprotein; Std Dev: Standard deviation; TBP: TATA-binding protein; TUB: Tubulin; YWHAZ: Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta.

Citation: Ferreira DB, Gasparoni LM, Bronzeri CF, Paiva KBS. RPLP0/TBP are the most stable reference genes for human dental pulp stem cells under osteogenic differentiation. World J Stem Cells 2024; 16(6): 656-669
URL: https://www.wjgnet.com/1948-0210/full/v16/i6/656.htm
DOI: https://dx.doi.org/10.4252/wjsc.v16.i6.656