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©The Author(s) 2024.
World J Stem Cells. Jun 26, 2024; 16(6): 656-669
Published online Jun 26, 2024. doi: 10.4252/wjsc.v16.i6.656
Published online Jun 26, 2024. doi: 10.4252/wjsc.v16.i6.656
Table 1 Candidate reference genes for quantitative real-time polymerase chain reaction
Gene symbol | Function | NCBI access | Primers sequences (5’-3’) | Temperature (°C) |
RPLP0 | Component of the 60S subunit | NM_001002 | F: AGCCCAGAACACTGGTCTC | 60 |
R: ACTCAGGATTTCAATGGTGCC | ||||
TBP | Transcription, metabolic pathways | NM_003194.5 | F: CACGAACCACGGCACTGATT | 62 |
R: TTTTCTTGCTGCCAGTCTGGA | ||||
YWHAZ | Signal transduction | NM_001135702 | F: TGATCCCCAATGCTTCACAAG | 61 |
R: GCCAAGTAACGGTAGTAATCTCC | ||||
RUNX2 | Master transcription factor for osteogenesis | NM_001015051 | F: TGGTTACTGTCATGGCGGGTA | 62 |
R: TCTCAGATCGTTGAACCTTGCTA | ||||
EF1α | Translation | NM_001402 | F: GAAGCTGGTATCTCCAAGAATGG | 61 |
R: CGACAATTAGTTGTTTCACACCC | ||||
GAPDH | Mitochondrial metabolism | NM_002046 | F: GCATCCTGGGCTACACTGA | 60 |
R: CCACCACCCTGTTGCTGTA | ||||
ACTB | Cytoskeleton | NM_001101 | F: CGACAGGATGCAGAAGGAG | 60 |
R: TCCTGCTTGCTGATCCACAT | ||||
B2M | Immune response | NM_004048 | F: GAGGCTATCCAGCGTACTCCA | 62 |
R: CGGCAGGCATACTCATCTTTT | ||||
ALAS1 | Mitochondrial metabolism | NM_199166 | F: AAATGAATGCCGTGAGGAAAGA | 60 |
R: CCCTCCATCGGTTTTCACACTA | ||||
TUB | Cytoskeleton | NM_001293212 | F: TCAACACCTTCTTCAGTGAAACG | 60 |
R: AGTGCCAGTGCGAACTTCATC | ||||
SDHA | Mitochondrial metabolism | NM_004168 | F: CAAACAGGAACCCGAGGTTTT | 60 |
R: CAGCTTGGTAACACATGCTGTAT |
- Citation: Ferreira DB, Gasparoni LM, Bronzeri CF, Paiva KBS. RPLP0/TBP are the most stable reference genes for human dental pulp stem cells under osteogenic differentiation. World J Stem Cells 2024; 16(6): 656-669
- URL: https://www.wjgnet.com/1948-0210/full/v16/i6/656.htm
- DOI: https://dx.doi.org/10.4252/wjsc.v16.i6.656