Effects of interleukin-10 treated macrophages on bone marrow mesenchymal stem cells via signal transducer and activator of transcription 3 pathway

Figure 7 The effect of interleukin-10 acting on macrophages after the inhibition of signal transducer and activator of transcription 3. A: Cell Counting Kit-8 assay of the bone marrow mesenchymal stem cells (BMSCs) proliferation after co-cultured with different groups of macrophages: M0, M0 + interleukin-10 (IL-10), M0 + IL-10 + Tyrphostin AG490 for 1, 3, 5, and 7 d; B: Real-time polymerase chain reaction (PCR) analysis for Alkaline phosphatase mRNA level in different groups of BMSCs (groups as above); C: Real-time PCR analysis for osteocalcin mRNA level in different groups of BMSCs (groups as above); D: Real-time PCR analysis for recombinant runt related transcription factor 2 mRNA level in different groups of BMSCs (groups as above). The expression levels were normalized to the expression of glyceraldehyde-3-phosphate dehydrogenase; E: Quantitative measurement of calcium mineral deposition was stained by Alizarin Red S staining after 14 d of osteogenic induction on BMSCs. n = 3 biological replicates. Data are represented as mean ± SD, statistically significant difference at the levels as ^aP < 0.05 and ^bP < 0.01. Alp: Alkaline phosphatase; Ocn: Osteocalcin; Runx2: Recombinant runt related transcription factor 2; Gapdh: Glyceraldehyde-3-phosphate dehydrogenase; IL-10: Interleukin-10.