Effects of interleukin-10 treated macrophages on bone marrow mesenchymal stem cells via signal transducer and activator of transcription 3 pathway

Figure 6 The effect of interleukin-10 treatment on the activation of signal transducer and activator of transcription 3 in macrophages. A: Western analysis of signal transducer and activator of transcription 3 (STAT3) and phosphorylated STAT3 protein in macrophages treated with/without interleukin-10. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) as a loading control. Tyrphostin AG490 was used at 10 μM concentration in culture medium as a phosphorylation specific inhibitor of STAT3. Data are representative of three independent experiments; B: Density values were measured by using Image J software for the representative blot shown. n = 3 biological replicates. Data are represented as mean ± SD, statistically significant difference at the levels as ^aP < 0.05 and ^bP < 0.01. IL-10: Interleukin-10; p-STAT3: Phosphorylated signal transducer and activator of transcription 3.