Effects of interleukin-10 treated macrophages on bone marrow mesenchymal stem cells via signal transducer and activator of transcription 3 pathway

Figure 3 The effect of macrophages on the proliferation and migration of bone marrow mesenchymal stem cells. A: Cell Counting Kit-8 assay of the 3^rd generation of bone marrow mesenchymal stem cells (BMSCs) proliferation after co-cultured with different groups of macrophages: M0, M0 + lipopolysaccharide (LPS), M0 + LPS + interleukin-10; compared with the control group for 1, 3, 5, and 7 d; B: Migration analysis of BMSCs and those co-cultured with different groups of macrophages (groups as above). The Image J software was adopted to process the pictures; C: Quantitative analysis of residual scratch area to initial scratch area percentage. Macrophages influenced the migration but not the proliferation of bone marrow mesenchymal stem cells. IL-10: Interleukin-10; LPS: Lipopolysaccharide.