Effects of interleukin-10 treated macrophages on bone marrow mesenchymal stem cells via signal transducer and activator of transcription 3 pathway

Figure 2 The effect of interleukin-10 acting on the macrophages on the inflammatory reaction environment. A: Real-time polymerase chain reaction (PCR) analysis for inducible nitric oxide synthase mRNA level in different groups of macrophages; B: Real-time PCR analysis for tumor necrosis factor-αmRNA level in different groups of macrophages; C: Real-time PCR analysis for Arginase 1 mRNA level in different groups of macrophages; D: Real-time PCR analysis for interleukin-10 mRNA level in different groups of macrophages. M0 group was stimulated without any special treatment. M0 + lipopolysaccharide (LPS) group was stimulated with LPS (1 μg/mL) for 12 h, then cultured the cells in normal medium for another 12 h. M0 + LPS + interleukin-10 group was stimulated with LPS (1 μg/mL) for 12 h, then stimulated with interleukin-10 (100 ng/mL) for another 12 h. The expression levels were normalized to the expression of glyceraldehyde-3-phosphate dehydrogenase (Gapdh). n = 3 biological replicates. Data are represented as mean ± SD, statistically significant difference at the levels as ^aP < 0.05 and ^bP < 0.01. Arg1: Arginase 1; Il10: Interleukin-10; Inos: Inducible nitric oxide synthase (gene); Tnfa: Tumor necrosis factor-α; Gapdh: Glyceraldehyde-3-phosphate dehydrogenase; LPS: Lipopolysaccharide.