GATA binding protein 2 mediated ankyrin repeat domain containing 26 high expression in myeloid-derived cell lines

Figure 3 The expression of ankyrin repeat domain containing 26 is upregulated by the overexpression of GATA binding protein 2. A: Agarose gels after polymerase chain reaction (PCR) to assess the expression of GATA binding protein 2 (GATA2) in HEL, K562, human induced pluripotent stem cells derived from bone marrow (hiPSC-BM) and human induced pluripotent stem cells derived from urothelium (hiPSC-U); B: Quantitative real-time PCR (RT-qPCR) analyses showing the promoting effect of GATA2 on ankyrin repeat domain containing 26 (ANKRD26) mRNA expression. K562 cells were transfected with GATA2 plasmids and control plasmids; C: Western blots showing the effect of GATA2 on promoting ANKRD26 protein expression in K562 cells; D: RT-qPCR analyses showing the effect of GATA2 on promoting ANKRD26 mRNA expression. HEL cells were transfected with GATA2 plasmids and control plasmids; E: Western blots showing the effect of GATA2 on promoting ANKRD26 expression at the protein level in HEL cells. The error bars represent the means ± SD of triplicate samples. ^bP < 0.01, calculated by Student’s t test. GATA2: GATA binding protein 2; ANKRD26: Ankyrin repeat domain containing 26; RUNX1: Runt related transcription factor 1; FLI1: Friend leukemia integration 1; hiPSC-BM: Human induced pluripotent stem cells derived from bone marrow; hiPSC-U: Human induced pluripotent stem cells derived from urothelium.