Patient-derived induced pluripotent stem cells with a MERTK mutation exhibit cell junction abnormalities and aberrant cellular differentiation potential

Figure 3 Abnormal adherent capability and aberrant differentiation potential. A and B: Imaging of clones floating on the surface of the culture medium by phase-contrast microscopy. Scale bar = 1000 μm; C and D: Imaging of cells during epithelial cell differentiation. Scale bar = 200 μm (C), scale bar = 400 μm (D); E: Cell counting kit-8 detection of human induced pluripotent stem cell proliferation ability. mean ± SEM, n = 12-14; F: Quantitative polymerase chain reaction analysis of representative cadherin subfamily members CELSR2 and DCHS1. mean ± SEM, n = 5. Two-tailed t-test. ^aP < 0.05, ^bP < 0.05. NS: No significance. ESC: Embryonic stem cell.