Sinomenine increases osteogenesis in mice with ovariectomy-induced bone loss by modulating autophagy

Figure 3 Sinomenine promotes osteogenic differentiation of bone marrow mesenchymal stromal cells but can be reversed by 3-methyladenine. A: Representative images of ALP staining at 7 d; B: Representative images of Alizarin red S (ARS) staining at 21 d; C and D: Quantitative analysis of ALP activity and the ARS recovery ratio; E-H: Protein levels of ALP, RUNX2 and COL1A1 after sinomenine (SIN) and 3-methyladenine treatment for five days; I-K: Runx2, ALP and COL1A1 mRNA levels after SIN treatment for five days. n = 3 per group. The three groups were cultured in osteogenic medium, and the values are shown as the mean ± SD. ^aP < 0.01, all data were normalized relative to the sham bone marrow mesenchymal stromal cell group. OVX: Ovariectomized; SIN: Sinomenine; 3-MA: 3-methyladenine; BMSC: Bone marrow mesenchymal stromal cell.