Effects of interleukin-10 treated macrophages on bone marrow mesenchymal stem cells via signal transducer and activator of transcription 3 pathway

doi:10.4252/wjsc.v16.i5.560

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World Journal of Stem Cells

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1948-0210

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Stem Cells. May 26, 2024; 16(5): 560-574
Published online May 26, 2024. doi: 10.4252/wjsc.v16.i5.560

Table 2 The real-time fluorescence quantitative polymerase chain reaction primer sequences for differentiation of bone marrow mesenchymal stem cells detection

Primer	Sequences
Gapdh	Forward: 5’-TATGACTCTACCCACGGCAA-3’
Gapdh	Reverse: 5’-ATACTCAGCACCAGCATCACC-3’
Alp	Forward: 5’-AACGTGGCCAAGAACATCATCA-3’
Alp	Reverse: 5’-TGTCCATCTCCAGCCGTGTC-3’
Ocn	Forward: 5’-AAAGCCCAGCGACTCT-3’
Ocn	Reverse: 5’-CTAAACGGTGGTGCCATAGAT-3’
Runx2	Forward: 5’-GCTTCTCCAACCCACGAATG-3’
Runx2	Reverse: 5’-GAACTGATAGGACGCTGACGA-3’

Relative mRNA expression levels were analyzed using the 2^-∆∆Ct method with glyceraldehyde-3-phosphate dehydrogenase as an internal reference. Alp: Alkaline phosphatase; Ocn: Osteocalcin; Runx2: Recombinant runt related transcription factor 2; Gapdh: Glyceraldehyde-3-phosphate dehydrogenase.

Citation: Lyu MH, Bian C, Dou YP, Gao K, Xu JJ, Ma P. Effects of interleukin-10 treated macrophages on bone marrow mesenchymal stem cells via signal transducer and activator of transcription 3 pathway. World J Stem Cells 2024; 16(5): 560-574
URL: https://www.wjgnet.com/1948-0210/full/v16/i5/560.htm
DOI: https://dx.doi.org/10.4252/wjsc.v16.i5.560