Gossypol acetic acid regulates leukemia stem cells by degrading LRPPRC via inhibiting IL-6/JAK1/STAT3 signaling or resulting mitochondrial dysfunction

Figure 7 Gossypol acetic acid positively regulates forkhead box M1 in and interleukin 6/janus kinase 1/signal transducer and activator of transcription 3 signaling independent manner. A: By employing GEPIA online tool, the correlation between leucine-rich pentatricopeptide repeat-containing protein (LRPPRC) and forkhead box M1 (FOXM1) in whole blood, acute myeloid leukemia cell lines and LAML samples were analyzed; B: After efficiently knockdown LRPPRC in KG1a-SCs and Kasumi-1-SCs, LRPPRC and FOXM1 protein levels were measured by performing western blot. ^aP < 0.05, vs shScram group; C: After being cultured with gossypol acetic acid (GAA), or pharmacology inhibitor S3I-210 for 24 h, FOXM1 and its downstream target genes, including PRDX3, MnSOD and catalase, were measured by performing western blot. ^aP < 0.05, vs GAA(-)/S3I-210(-) group. LRPPRC: Leucine-rich pentatricopeptide repeat-containing protein; GAA: Gossypol acetic acid; FOXM1: Forkhead box M1.