Gossypol acetic acid regulates leukemia stem cells by degrading LRPPRC via inhibiting IL-6/JAK1/STAT3 signaling or resulting mitochondrial dysfunction

Figure 5 Gossypol acetic acid treatment inhibited interleukin 6/janus kinase 1/signal transducer and activator of transcription 3 signaling. A: After being treated with 5-50 ng/mL interleukin 6 (IL-6) for 6 h, western blot was performed to detect IL-6/janus kinase 1/signal transducer and activator of transcription 3 (IL-6/JAK1/STAT3) signaling including STAT3, p-STAT3, JAK1, p-JAK1; B: After being pre-treated with gossypol acetic acid for 24 h, 10 ng/mL IL-6 was added for 6-hour extra incubation and IL-6/JAK1/STAT3 signaling including STAT3, p-STAT3, JAK1, p-JAK1 was detected. ^aP < 0.05, vs IL-6 (-) group; ^bP < 0.05, vs IL-6 (+) group. LRPPRC: Leucine-rich pentatricopeptide repeat-containing protein; GAA: Gossypol acetic acid; IL-6: Interleukin 6; JAK1: Janus kinase 1; STAT3: Signal transducer and activator of transcription 3.