Gossypol acetic acid regulates leukemia stem cells by degrading LRPPRC via inhibiting IL-6/JAK1/STAT3 signaling or resulting mitochondrial dysfunction

Figure 3 Gossypol acetic acid treatment enhanced chemosensitivity and apoptosis after chemotreatment. A: 0.5, 1, 2 or 4 μmol/L of cytarabine (Ara-C), Dexamethasone (Dex) or L-Asparaginase (L-Asp) was used for chemotreatment with or without presence of 5 μmol/L of gossypol acetic acid (GAA), 24-h later, cell viability was measured by performing CCK-8 assay. ^aP < 0.05, vs mock group; B: KG1a-SCs or Kasumi-1-SCs was co-cultured with 1 μmol/L of Ara-c, Dex or L-Asp with or without 5 μmol/L of GAA for 24 h, and cleaved-caspase 3 and cleaved-PARP were measured by performing western blot. LRPPRC: Leucine-rich pentatricopeptide repeat-containing protein; GAA: Gossypol acetic acid; Ara-C: Cytarabine; Dex: Dexamethasone; L-Asp: L-Asparaginase.