Gossypol acetic acid regulates leukemia stem cells by degrading LRPPRC via inhibiting IL-6/JAK1/STAT3 signaling or resulting mitochondrial dysfunction

Figure 2 Leucine-rich pentatricopeptide repeat-containing protein knockdown decreased cell viability and colony formation in KG1a-SCs and Kasumi-1-SCs. A: After magnetic sorting, sorted or original cells were analyzed by flow cytometry after double staining using CD34-PE/CD38-FITC. CD34⁺/CD38^- proportion was considered as leukemia stem cell subpopulation. ^aP < 0.05, vs KG1a group; ^bP < 0.05, vs Kasumi-1 group; B: Leucine-rich pentatricopeptide repeat-containing protein (LRPPRC) expression was measured by performing western blot. ^aP < 0.05, vs KG1a group; ^bP < 0.05, vs Kasumi-1 group; C: After short hairpin RNA (shRNA) transfection targeting to LRPPRC mRNA for 48 h, LRPPRC protein level was measured. ^aP < 0.05, vs KG1a group or Kasumi-1 group; ^bP < 0.05, vs Kasumi-1 group; D: Cell viability was measured from day 1 to 5 after LRPPRC knockdown (shLRPPRC group). ^aP < 0.05, vs KG1a-SCs shScram group; ^bP < 0.05, vs Kasumi-1-SCs shScram group; E: Colony formation assay was performed to evaluate the effect of LRPPRC knockdown. LRPPRC: Leucine-rich pentatricopeptide repeat-containing protein.