Gossypol acetic acid regulates leukemia stem cells by degrading LRPPRC via inhibiting IL-6/JAK1/STAT3 signaling or resulting mitochondrial dysfunction

Figure 1 Gossypol acetic acid decreased leucine-rich pentatricopeptide repeat-containing protein and inhibited leukemia stem cell progression. A: Molecular structure of gossypol acetic acid (GAA) was presented; B: After being treated with 2.5, 5, 7.5, 10 μmol/L GAA for 24 h, leucine-rich pentatricopeptide repeat-containing protein was measured by performing western blot; C: After being cultured with 2.5, 5, 7.5, 10 μmol/L GAA for 24 h, cell viability was measured by performing CCK-8 assay. ^aP < 0.05, vs KG1a-SCs; ^bP < 0.01, vs KG1a-SCs; ^cP < 0.05, vs Kasumi-1-SCs; ^dP < 0.01, vs Kasumi-1-SCs; D: With presence of 2.5, 5, 7.5, 10 μmol/L GAA, colony formation assay was performed. LRPPRC: Leucine-rich pentatricopeptide repeat-containing protein; GAA: Gossypol acetic acid.