Expansion of human umbilical cord derived mesenchymal stem cells in regenerative medicine

Figure 13  Mycoplasma, cytomegalovirus, and endotoxin analysis. A and B: The normalized gene expression of mycoplasma and cytomegalovirus in recultured human umbilical cord-mesenchymal stem cells (hUC-MSCs): To evaluate the normalized gene expression of Mycoplasma hominis (M. hominis) and M. arginini and cytomegalovirus, quantification was performed by real-time polymerase chain reaction in triplicate at passages 1-6. The X-axis represents the MSCs derived from recultured hUC at three recultured ranges and the Y-axis represents M. hominis and M. arginini and cytomegalovirus expression normalized to housekeeping gene hydroxymethyl-bilane synthase. The expression at each recultured group showed negative expression of both strains of Mycoplasma. Values are presented as mean ± SD from six independent experiments. Statistically non-significant normalized expression was obtained; C: Recultured hUC-MSCs endotoxin analysis: The level of endotoxin in recultured hUC-MSC was measured by using the Pierce LAL Chromogenic Endotoxin Quantification Kit. The concentration of endotoxin is below 0.5 EU/mL. hUC: Human umbilical cord; MSC: Mesenchymal stem cell.