Expansion of human umbilical cord derived mesenchymal stem cells in regenerative medicine

Figure 11  Colony forming unit assay. A: Formation of colonies at recultured human umbilical cord-mesenchymal stem cells (hUC-MSCs) number: Representation of phase contrast microscopy of spindle-shaped rapidly proliferated colonies of recultured hUC-MSCs at each recultured number (R1-R10); B: Analysis of recultured hUC-MSCs by colony forming unit (CFU): Statistical analysis showed no significant difference in CFU at each recultured hUC-MSCs; all the recultured population of MSCs showed self-replicated spindle-shaped cells that possess the proficiency to form discrete colonies. The experiments were performed in triplicates. NS: No significance. hUC: Human umbilical cord; MSC: Mesenchymal stem cell.