Expansion of human umbilical cord derived mesenchymal stem cells in regenerative medicine

Figure 9 The proliferation gene expression analysis of recultured human umbilical cord-mesenchymal stem cells. The three different ranges of recultured human umbilical cord derived mesenchymal stem cells (MSCs) were analyzed to evaluate gene expression of proliferation markers of MSCs. The normalized mRNA expression of paired-box 6, bone morphogenetic protein 2, and transforming growth factor β1 showed no significant difference at recultured 1-4, 5-7, and 8-10. GAPDH was used as a housekeeping gene to normalize the gene expression. NS: No significance. BMP2: Bone morphogenetic proteins; PAX6: Paired-box 6; TGFβ1: Transforming growth factor beta1; hUC: Human umbilical cord.