Expansion of human umbilical cord derived mesenchymal stem cells in regenerative medicine

Figure 6 Trilineage differentiation of mesenchymal stem cells. A: The differentiation of recultured human umbilical cord-mesenchymal stem cells (hUC-MSCs) towards osteocytes, the stained cells showed the presence of calcium and minerals deposits by Alizarin red staining as compared to the control cells; B: The chondrogenic differentiation of recultured hUC-MSCs, was confirmed by Alcian blue stained cells showed showing the presence of glycosaminoglycan and proteoglycan in comparison to the control cells; C: The hUC-MSCs differentiated into adipocytes were confirmed by staining the cells with Oil red O, the accumulated lipid droplets indicated the differentiation of recultured hUC-MSCs towards adipogenic lineage, compared to the control cells.