Expansion of human umbilical cord derived mesenchymal stem cells in regenerative medicine

Figure 5 Immuno-cytochemical profiling of recultured human umbilical cord-mesenchymal stem cells. A: The recultured human umbilical cord (hUC)-mesenchymal stem cells (MSCs) were positive for CD73, CD105, vimentin, CD29, CD90, Stro-1, and Lin28. The cells were stained with specific antibodies against MSCs markers. Nuclei were stained with DAPI. The cells were visualized for F-actin by staining with phalloidin labeled with Alexa Flour 488; B: The hUC-MSCs were negative for CD45 and HLA-DR MSCs negative markers by immunocytochemical staining.