Unveiling the role of hypoxia-inducible factor 2alpha in osteoporosis: Implications for bone health

Figure 2 Hypoxia-inducible factor 2alpha-deficient mice were grossly developmentally normal and exhibited mild anemia but normal hematopoiesis. A: Representative images of two-month-old Prx1-Cre;Hif-2α^fl/fl and Hif-2α^fl/fl mice; B: Body masses of 2-month-old Prx1-Cre;Hif-2α^fl/fl and Hif-2α^fl/fl mice; C: Total nucleated cells in bone marrow (BM); D-O: BM cells freshly harvested from 8-wk-old Prx1-Cre;Hif-2α^fl/fl and Hif-2α^fl/fl mice were assayed by multiparametric fluorescence-activated cell sorting (FACS) analyses to determine the frequencies of hematopoietic stem cells (HSCs) (D), HSC numbers (E), frequencies of progenitors (G), and progenitor numbers (H); BM cells harvested from Prx1-Cre;Hif-2α^fl/fl and Hif-2α^fl/fl mice were assayed by FACS to determine the cell cycle status of HSCs. The percentages of HSCs in G0, G1, and S/G2/M phases (F) were quantified; erythrocyte count (I), hemoglobin content (J), hematocrit value (K), white blood cell count (L), platelet count (M), neutrophil count (N), and lymphocyte count (O) in peripheral blood of Prx1-Cre;Hif-2α^fl/fl and Hif-2α^fl/fl mice; ^aP < 0.05. TNC: Total nucleated cell; HSC: Hematopoietic stem cell; LT-HSC: Long-term hematopoietic stem cell; ST-HSC: Short-term hematopoietic stem cell; MPP: Multipotent progenitor; MSC: Mesenchymal stem cell; CMP: Common myeloid progenitor; GMP: Granulocyte-monocyte progenitor; MEP: Megakaryocyte-erythroid progenitor; CLP: Common lymphoid progenitor; RBC: Red blood cell; HGB: Hemoglobin; HCT: Hematocrit; WBC: White blood cell; PLT: Platelet; NEU: Neutrophil; LYMPH: Lymphocyte; HIF-2α: Hypoxia-inducible factor 2alpha.