Expansion of human umbilical cord derived mesenchymal stem cells in regenerative medicine

Table 1 Mesenchymal stem cell specific antibodies used in immunocytochemistry

Primary antibody markers	Molecular function and binding	Working dilution	Manufacturer information
CD73	Hyaluronan receptor	1:100	550256, BD Pharmingen, United States
CD105	Endoglin	1:100	560839, BD Pharmingen, United States
Vimentin	Epithelial-mesenchymal transition	1:100	V6389, Sigma-Aldrich, United States
CD29	Membrane glycoprotein	1:100	MAB-1981, Chemicon International, United States
CD90	Thymocyte antigen 1	1:100	CL005AP, EMD Millipore Corp., United States
Stro-1	Mesenchymal cell precursor marker	1:100	14-6688-82, Molecular Probes, Invitrogen, United States
Lin28	Cell surface MSC marker	1:100	PA1-096, Molecular Probes, Invitrogen, United States
CD45	Lymphocyte antigen	1:100	CBL415, BD Pharmingen, United States
HLA-DR	MHC class II immunogenic marker	1:100	14-9956-82, Molecular Probes, Invitrogen, United States
Secondary antibody	Conjugated label	Working dilution	Manufacturer information
Goat anti-rabbit IgG	Alexa Fluor 546	1:200	A-11010 Molecular Probes, Invitrogen, United States
Goat anti-mouse IgG	Alexa Fluor 546	1:200	A-11030 Molecular Probes, Invitrogen, United States

MHC: Major histocompatibility complex; IgG: Immunoglobulin G.