Unveiling the role of hypoxia-inducible factor 2alpha in osteoporosis: Implications for bone health

doi:10.4252/wjsc.v16.i4.389

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Apr 26, 2024 (publication date) through Dec 27, 2024

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World Journal of Stem Cells

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1948-0210

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Stem Cells. Apr 26, 2024; 16(4): 389-409
Published online Apr 26, 2024. doi: 10.4252/wjsc.v16.i4.389

Table 2 Primers used for quantitative real-time polymerase chain reaction of total RNA

Genes	Primers types	Sequence (5’-3’)
HIF-2α	Forward	CTCCCACCTGGACAAAGC
HIF-2α	Reverse	GTCGCAAGGATGAGTGAAGT
HIF-2α exon2	Forward	ACAAAGCCTCCATCATGCG
HIF-2α exon2	Reverse	CGTCTTGGGTCACCACAG
Runx2	Forward	TCCACAAGGACAGAGTCAGATTACAG
Runx2	Reverse	CAGAAGTCAGAGGTGGCAGTGTCATC
Col1α1	Forward	TGTGTGCGATGACGTGCAAT
Col1α1	Reverse	GGGTCCCTCGACTCCTACA
OCN	Forward	AGACTCCGGCGCTACCTT
OCN	Reverse	AAGCAGGGTCAAGCTCACAT
CEBPa	Forward	CAAGAACAGCAACGAGTACCG
CEBPa	Reverse	GTCACTGGTCAACTCCAGCAC
Lpl	Forward	GCGTAGCAGGAAGTCTGACCAA
Lpl	Reverse	AGCGTCATCAGGAGAAAGGCGA
Adipo-nectin	Forward	TGTTCCTCTTAATCCTGCCCA
Adipo-nectin	Reverse	CCAACCTGCACAAGTTCCCTT
PI3K	Forward	ACACCACGGTTTGGACTATGG
PI3K	Reverse	GGCTACAGTAGTGGGCTTGG
AKT	Forward	TGGGTCAAGGAACAGAAGCA
AKT	Reverse	TCACACTGACCACTGACACA
mTOR	Forward	CGGGACTCTTTACACTGCG
mTOR	Reverse	CCTTCAGGCTCAACCAACA
Actin	Forward	TTGCTGACAGGATGCAGAAGGAGA
Actin	Reverse	ACTCCTGCTTGCTGATCCACATCT

HIF-2α: Hypoxia-inducible factor 2alpha; mTOR: Mechanistic target of rapamycin.

Citation: Wang LL, Lu ZJ, Luo SK, Li Y, Yang Z, Lu HY. Unveiling the role of hypoxia-inducible factor 2alpha in osteoporosis: Implications for bone health. World J Stem Cells 2024; 16(4): 389-409
URL: https://www.wjgnet.com/1948-0210/full/v16/i4/389.htm
DOI: https://dx.doi.org/10.4252/wjsc.v16.i4.389