Low-intensity pulsed ultrasound reduces alveolar bone resorption during orthodontic treatment via Lamin A/C-Yes-associated protein axis in stem cells

Figure 7 siYAP1 blocks the effect of low-intensity pulsed ultrasound-induced osteogenic differentiation of bone marrow mesenchymal stem cells. A: Representative immunofluorescence images of Yes-associated protein (YAP1) (red) and DAPI (blue) staining (left) and statistical analyses (right); B: Western blotting results for type 1 collagen, YAP1, runt-related transcription factor 2 (RUNX2), and alkaline phosphatase (ALP); C: mRNA expression of ALP, RUNX2, and YAP1 in the force group by quantitative reverse transcription-polymerase chain reaction; D and E: Representative ALP and alizarin red staining staining images and statistical analysis. ^aP < 0.05 vs control group, ^bP < 0.01 vs control group. ALP: Alkaline phosphatase; COL1: Type 1 collagen; LIPUS: Low-intensity pulsed ultrasound; RUNX2: Runt-related transcription factor 2; YAP1: Yes-associated protein; ARS: Alizarin red staining.