Low-intensity pulsed ultrasound reduces alveolar bone resorption during orthodontic treatment via Lamin A/C-Yes-associated protein axis in stem cells

Figure 4 Low-intensity pulsed ultrasound accelerates tooth movement, inhibits alveolar bone resorption, and promotes bone formation. A: Schematic diagram of the animal experiment procedure. The orthodontic tooth movement model was established on day 0. From day 1 to the end point, the force + low-intensity pulsed ultrasound (LIPUS) group was stimulated with LIPUS for 20 min every day until the samples were harvested on days 7 and 14; B: Schematic representation of LIPUS stimulating the maxillary first molar at the corresponding position on the buccal side; C-F: Representative images (C) and 3D reconstructed model images (E). Tooth movement distances (D) and distances from the alveolar crest to the cementum-enamel boundary (F) were measured with Mimics software; G-I: Representative region of interest (ROI) region selection diagrams and statistical analysis of the BV/TV, Tb.Th, Tb.N, Tb.Sp, and BS/BV values of the ROI. ^aP < 0.05 vs control group, ^bP < 0.01 vs control group. CT: Computed tomography; LIPUS: Low-intensity pulsed ultrasound.