Effects of different concentrations of nicotinamide on hematopoietic stem cells cultured in vitro

Figure 1 Proportion of hematopoietic stem and progenitor cells, short-term repopulating hematopoietic stem cells and long-term repopulating hematopoietic stem cells populations in each group of cultured cells. A: Phenotype (up) and proportion (down) of hematopoietic stem and progenitor cells; B: Phenotype (up) and proportion (down) of short-term repopulating hematopoietic stem cells (ST-HSCs); C: Phenotype (up) and proportion (down) of long-term repopulating HSCs; D: Cell size for each group of cells; E: Viability of each group of cells; F: Typical images of representative morphologies of various type of colonies as indicated; G: Colony formation assay showing the number of various types of cell colonies as indicated. The number of colony-forming unit (CFU)-erythroid, CFU-macrophage and CFU-granulocyte/erythroid/macrophage/megakaryocyte were scored. ^aP < 0.05; ^bP < 0.01; ^cP < 0.001; ^dP < 0.0001; NS: Not significant. NAM: Nicotinamide; CFU-E: Colony-forming unit-erythroid; CFU-GM: Colony-forming unit-granulocyte, macrophage; CFU-GEMM: Colony-forming unit-granulocyte/erythroid/macrophage/megakaryocyte.