Silencing of Jumonji domain-containing 1C inhibits the osteogenic differentiation of bone marrow mesenchymal stem cells via nuclear factor-κB signaling

Figure 4 Jumonji domain-containing 1C was downregulated after bone marrow-derived macrophages osteoclast differentiation. Bone marrow-derived macrophages were incubated with RANKL for osteoclast differentiation. A: Representative images of tissue-resistant acid phosphatase staining were presented; B-D: Jumonji domain-containing 1C mRNA and protein levels in MMCs after osteoclast induction were measured by reverse transcription coupled to the quantitative polymerase chain reaction and western blot. All values are shown as mean ± SD. ^aP < 0.05, ^dP < 0.0001, n = 3. JMJD1C: Jumonji domain-containing 1C.