Silencing of Jumonji domain-containing 1C inhibits the osteogenic differentiation of bone marrow mesenchymal stem cells via nuclear factor-κB signaling

Figure 3 Jumonji domain-containing 1C knockdown inhibited osteogenic differentiation and upregulated p-nuclear factor-κB expression in bone mesenchymal stem cells. Bone mesenchymal stem cells (BMSCs) were transfected with sh-NC or short hairpin RNA against Jumonji domain-containing 1C (sh-JMJD1C). A-C: Western blot was adopted to measure JMJD1C and p-nuclear factor-κB protein levels; D and E: Detection of osteogenic differentiation ability of BMSCs using alizarin red staining; F-H: Cytokines secretion level of interleukin (IL)-1β, IL-6 and tumor necrosis factor alpha were detected by enzyme-linked immunosorbent assay. All values are shown as mean ± SD. ^bP < 0.01, ^cP < 0.001, ^dP < 0.0001, n = 3. JMJD1C: Jumonji domain-containing 1C; TNF-α: Tumor necrosis factor alpha; IL: Interleukin; NF-κB: nuclear factor-κB.