Silencing of Jumonji domain-containing 1C inhibits the osteogenic differentiation of bone marrow mesenchymal stem cells via nuclear factor-κB signaling

Figure 2 Jumonji domain-containing 1C was upregulated while nuclear factor-κB activation were not affected during bone mesenchymal stem cells osteogenic differentiation. A: Jumonji domain-containing 1C (JMJD1C) mRNA level in bone mesenchymal stem cells after osteoblast induction was measured by reverse transcription coupled to the quantitative polymerase chain reaction; B-D: The protein expressions of JMJD1C and p-nuclear factor-κB were determined by western blot; E-G: The levels of inflammatory cytokines [interleukin (IL)-1β, IL-6 and tumor necrosis factor alpha] were detected using enzyme-linked immunosorbent assay. All values are shown as mean ± SD. ^aP < 0.05, ^dP < 0.0001, NS: No significance. n = 3. JMJD1C: Jumonji domain-containing 1C; TNF-α: Tumor necrosis factor alpha; IL: Interleukin; NF-κB: nuclear factor-κB.