Preliminary study on the preparation of lyophilized acellular nerve scaffold complexes from rabbit sciatic nerves with human umbilical cord mesenchymal stem cells

Figure 2 Histological observation of acellular scaffolds. A: Hematoxylin and eosin (HE) staining shows the peripheral nerve fibers enveloped by the perineurium and a large amount of nuclear components may be seen in the peripheral nerves; B: After decellularization, the matrix structure of the HE-stained nerve fibers is preserved; however, the cell structure is no longer visible; C: Luxol fast blue (LFB) staining of peripheral nerve tissue reveals significant blue staining of myelin sheath tissue; D: LFB staining shows decreased staining of decellularized nerve tissue and myelin sheaths.