Exosomes derived from microRNA-540-3p overexpressing mesenchymal stem cells promote immune tolerance via the CD74/nuclear factor-kappaB pathway in cardiac allograft

Figure 7 CD74 is a target of microRNA-540-3p and regulates P65 expression. A: Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analyses of microRNA-540-3p (miR-540-3p) targets; B: A dual-luciferase assay was used to detect the binding of miR-540-3p to CD74; C: Co-IP was used to detect the binding of CD74 and nuclear factor-kappaB (NF-κB) in dendritic cells (DCs); D: Co-IP was used to detect the binding of CD74 and NF-κB in T cells; E-G: Western blot analysis of the expression of CD74 and NF-κB in DCs; H-J: Western blot analysis of the expression of CD74 and NF-κB in T cells; K-M: Western blot analysis of CD74 and NF-κB expression in DCs after co-culture with T cells; N-P: Western blot analysis of the expression of CD74 and NF-κB in T cells after co-culture with DCs; Q-S: Western blot analysis of the expression of CD74 and NF-κB in DCs after plasmid transfection; T-V: Western blot analysis of CD74 and NF-κB expression in T cells after plasmid transfection. ^aP < 0.05, ^bP < 0.01, ^cP < 0.001, ^dP < 0.0001. Mut: Mutant; BMSC: Bone marrow mesenchymal stem cell; IDO: Indoleamine 2,3-dioxygenase; DC: Dendritic cell; LPS: Lipopolysaccharide; exo: Exosomes; NF-κB: Nuclear factor-kappaB.