Exosomes derived from microRNA-540-3p overexpressing mesenchymal stem cells promote immune tolerance via the CD74/nuclear factor-kappaB pathway in cardiac allograft

Figure 6 Effect of bone marrow mesenchymal stem cell^{miR-540-3p-mimic} exosomes on dendritic cells after co-culturing with T cells. A-D: Flow cytometry analysis of the positive rate of cell surface markers (major histocompatibility complex II, CD80, CD86, and CD274) in dendritic cells cultured with T cells for 72 hours; E-G: Flow cytometry analysis of the positive rate of T cells (CD4⁺ T, CD8⁺ T, and T regulatory cells) and T cells after co-culture with dendritic cells; H-K: Enzyme-linked immunosorbent assay-based quantitative analysis of cytokine production (interleukin-1β, interferon-γ, interleukin-10, and transforming growth factor β1) in dendritic cell-T cells co-cultures. ^aP < 0.05, ^dP < 0.0001. MHC: Major histocompatibility complex; BMSC: Bone marrow mesenchymal stem cell; IDO: Indoleamine 2,3-dioxygenase; DC: Dendritic cell; LPS: Lipopolysaccharide; exo: Exosomes; IL: Interleukin; TGF: Transforming growth factor; IFN: Interferon; Treg: T regulatory cell.