Exosomes derived from microRNA-540-3p overexpressing mesenchymal stem cells promote immune tolerance via the CD74/nuclear factor-kappaB pathway in cardiac allograft

Figure 4 Bone marrow mesenchymal stem cell^{miR-540-3p-mimic} exosomes reduces immune resistance in dendritic cells. A-D: Flow cytometry analysis of the positive rate of cell surface markers in dendritic cells (major histocompatibility complex II, CD80, CD86, and CD274) for 72 hours; E-H: Enzyme-linked immunosorbent assay-based quantitative analysis of cytokine production (interleukin-1β, interferon-γ, interleukin-10, transforming growth factor β1) in dendritic cells. ^aP < 0.05, ^dP < 0.0001. MHC: Major histocompatibility complex; BMSC: Bone marrow mesenchymal stem cell; IDO: Indoleamine 2,3-dioxygenase; DC: Dendritic cell; LPS: Lipopolysaccharide; exo: Exosomes; IL: Interleukin; TGF: Transforming growth factor; IFN: Interferon.