Exosomes derived from microRNA-540-3p overexpressing mesenchymal stem cells promote immune tolerance via the CD74/nuclear factor-kappaB pathway in cardiac allograft

Figure 2 Overexpression of microRNA-540-3p in T cells modulates cell subtypes and cytokine production. A: Expression of microRNA-540-3p in T cells at 48, 72, and 96 hours post-plasmid transfection; B-D: Flow cytometry analysis of the positive rate of T cells (CD4⁺ T, CD8⁺ T, and T regulatory cell) and T cells after co-culture with dendritic cells for 72 hours; E-H: Enzyme-linked immunosorbent assay-based quantitative analysis of cytokine production (interleukin-1β, interferon-γ, interleukin-10, and transforming growth factor β1) in T cells and T cells after co-culture with dendritic cells for 72 hours. ^aP < 0.05, ^dP < 0.0001. NC: Negative control; LPS: Lipopolysaccharide; IL: Interleukin; TGF: Transforming growth factor; IFN: Interferon.