Exosome-based strategy against colon cancer using small interfering RNA-loaded vesicles targeting soluble a proliferation-inducing ligand

Figure 5 Anticancer effects of small interfering peptidyl-prolyl cis-trans isomerase NIMA-interacting 1 RNA-loaded soluble a proliferation-inducing ligand-targeted exosome in excised tumor tissues. Western blot analysis of excised tumor tissues revealed that the small interfering peptidyl-prolyl cis-trans isomerase NIMA-interacting 1 RNA-loaded soluble a proliferation-inducing ligand-targeted exosome (tEx[p]) group showed the lowest peptidyl-prolyl cis-trans isomerase NIMA-interacting 1 (P1N1) expression levels, coupled with the highest expression of the epithelial marker E-cadherin, and the lowest levels of mesenchymal markers Vimentin and Snail (P < 0.05), suggesting that tEx[p] effectively inhibits epithelial-mesenchymal transition in tumor cells. Relative densities of individual markers had been quantified using Image J software and then were normalized to that of β-actin in each group. Values are presented as mean ± standard deviation of three independent experiments. ^aP < 0.05, ^bP < 0.01. Ex: Exosome.