Exosome-based strategy against colon cancer using small interfering RNA-loaded vesicles targeting soluble a proliferation-inducing ligand

Figure 2 In vitro efficacy of small interfering peptidyl-prolyl cis-trans isomerase NIMA-interacting 1 RNA-loaded soluble a proliferation-inducing ligand-targeted exosome in HCT116 colon cancer cells. A: Western blot analysis demonstrating epithelial-mesenchymal transition-inhibiting ability of small interfering peptidyl-prolyl cis-trans isomerase NIMA-interacting 1 RNA-loaded soluble a proliferation-inducing ligand-targeted exosome (tEx[p]). The tEx[p] group exhibited a significant increase in the epithelial marker E-cadherin (P < 0.05) and a decrease in mesenchymal markers Snail and Vimentin in HCT colon cancer cells; B: Wound healing assay demonstrating the inhibition of cell migration by tEx[p]. The tEx[p] group showed a marked reduction in cell migration, indicating the most effective inhibition of cell movement among all groups (P < 0.05). Relative densities of individual markers had been quantified using ImageJ software and then were normalized to that of β-actin in each group. ^bP < 0.01. Ex: Exosome.