Effects of miR-214-5p and miR-21-5p in hypoxic endometrial epithelial-cell-derived exosomes on human umbilical cord mesenchymal stem cells

Figure 7 MiR-21-5p or miR-214-5p overexpression represses human umbilical cord mesenchymal stem cell migration and differentiation into endometrial epithelial cells, while signal transducer and activator of transcription 3 activator reverses these effects. Human umbilical cord mesenchymal stem cells were transfected with miR-214-5p or miR21-3p mimic, and treated with signal transducer and activator of transcription 3 activator (colivelin). A and B: Transwell assay for detecting human umbilical cord mesenchymal stem cell migration; C and D: Wound healing assay for measurement of cell migration; E and F: Western blotting for measuring CD9, CK19, vimentin, CD13, and phospho (p)-signal transducer and activator of transcription 3. n = 3. NS: No significant difference, ^aP < 0.05, ^bP < 0.01, ^cP < 0.001, ^dP < 0.0001. Data are described as the mean ± SD. HucMSC: Human umbilical cord mesenchymal stem cells; NC: Normal control; STAT3: Signal transducer and activator of transcription 3.