Effects of miR-214-5p and miR-21-5p in hypoxic endometrial epithelial-cell-derived exosomes on human umbilical cord mesenchymal stem cells

Figure 6 Signal transducer and activator of transcription 3 inhibitor reverses the effects induced by miR-21-5p or miR-214-5p inhibitor on human umbilical cord mesenchymal stem cell function. Human umbilical cord mesenchymal stem cells transfected with miR-21-5p or miR-214-5p inhibitor, and treated with signal transducer and activator of transcription 3 inhibitor (Stattic). A and B: Transwell assay for detecting human umbilical cord mesenchymal stem cells migration; C and D: Wound healing assay for detection of cell migration; E and F: Protein levels of CD9, CK19, vimentin, and CD13 determined by Western blotting; G and H: Western blotting for detecting signal transducer and activator of transcription 3 and phospho (p)-signal transducer and activator of transcription 3 protein levels. n = 3. ^aP < 0.05, ^bP < 0.01, ^cP < 0.001, ^dP < 0.0001. Data are described as the mean ± SD. HucMSC: Human umbilical cord mesenchymal stem cells; NC: Normal control; STAT3: Signal transducer and activator of transcription 3.