Effects of miR-214-5p and miR-21-5p in hypoxic endometrial epithelial-cell-derived exosomes on human umbilical cord mesenchymal stem cells

Figure 4 Silencing of miR-21-5p or miR-214-5p promotes human umbilical cord mesenchymal stem cell migration and differentiation into endometrial epithelial cells. MiR-21-5p/miR-214-5p inhibitor was transfected into human umbilical cord mesenchymal stem cells. A and B: MiR-21-5p and miR-214-5p levels measured by reverse transcription-quantitative polymerase chain reaction; C and D: Cell migration determined using Transwell assay; E and F: Wound healing assay for measurement of cell migration; G and H: Protein expression of CD9, CK19, vimentin, and CD13 measured using Western blotting; I and J: Immunofluorescence staining for detecting expression of CD9, CK19, CD13, and vimentin. n = 3. ^aP < 0.05, ^bP < 0.01, ^cP < 0.001, ^dP < 0.0001. Data are described as the mean ± SD. HucMSC: Human umbilical cord mesenchymal stem cells; NC: Normal control.