Gamma-aminobutyric acid enhances miR-21-5p loading into adipose-derived stem cell extracellular vesicles to alleviate myocardial ischemia-reperfusion injury via TXNIP regulation

Figure 4 MicroRNA-21-5p derived from gamma-aminobutyric acid-induced adipose-derived mesenchymal stem cells from inguinal adipose tissue regulate thioredoxin-interacting protein expression in mRNA degree. A: MicroRNA (miRNA) sequencing results of gamma-aminobutyric acid (GABA)-induced adipose-derived mesenchymal stem cells (ADSCs) from inguinal adipose tissue (IAT) vs extracellular vesicles (EVs)^IAT; B: mRNA sequencing results of neonatal mouse cardiomyocytes exposed to GABA-induced ADSCs from IAT; C: Binding sites predicted for the 3’ untranslated region (UTR) regions of thioredoxin-interacting protein and miR-21-5p; D: Validation of the binding between thioredoxin-interacting protein and miR-21-5p using a dual luciferase reporter gene assay. H/R: Hypoxia and reoxygenation; miRNA: MicroRNA; MUT: Mutated; NC: Negative control; WT: Wild-type.