Integrin beta 3-overexpressing mesenchymal stromal cells display enhanced homing and can reduce atherosclerotic plaque

Figure 2 Expression of integrin subunit beta 3 on human umbilical cord-derived mesenchymal stem cells. A: Western blot; B: Quantitative real-time polymerase chain reaction (qRT-PCR) analysis of Integrin subunit beta 3 (ITGB3) expression; C: Representative immunofluorescence image; D: Quantification of ITGB3 and green fluorescence protein staining in untransfected and transfected mesenchymal stem cells (MSCs); E: Cell viability of untransfected and transfected MSCs determined by CCK8 assay. The experiment was repeated three times; F and G: Analysis of cell surface markers on MSC^ITGB3 using flow cytometry to determine whether they have pluripotent characteristics; H: Osteogenic differentiation of MSC^ITGB3 identified using Alizarin red staining; I: Oil Red O staining shows differentiation of MSC^ITGB3 into adipocytes; J: qRT-PCR analysis of marker genes in induced osteoblasts; K: qRT-PCR analysis of marker genes in induced adipocyte. ^bP < 0.001. GFP: Green fluorescence protein; ITGB3: Integrin subunit beta 3; MSC: Mesenchymal stem cell; ORO: Oil Red O; qRT-PCR: Quantitative real-time polymerase chain reaction.