Interferon-γ priming enhances the therapeutic effects of menstrual blood-derived stromal cells in a mouse liver ischemia-reperfusion model

Figure 3 Interferon-γ-primed menstrual blood-derived stromal cells attenuated liver ischemia-reperfusion injury in the mouse short-term ischemia-reperfusion model. A: Representative images of histological liver sections stained with hematoxylin and eosin (hematoxylin and eosin staining, magnification 200 ×, images in the second row are partial magnifications of regions indicated by the red box); B: The degree of liver injury in each group was determined according to Suzuki’s injury score. The data are expressed as the means ± SEMs (n = 10/group); C: Representative images of liver sections from each group stained for caspase-3 to evaluate the apoptosis level (magnification 200 ×, images in the second row are partial magnifications of regions indicated by the red box); D: Representative liver sections from each group stained with TUNEL were obtained to evaluate the apoptosis level (magnification 200 ×, images in the second row are partial magnifications of regions indicated by the red box); E: The serum alanine aminotransferase levels were measured to indicate liver function levels, the data are expressed as the means ± SEMs (n = 3/group); F: The serum aspartate aminotransferase levels were measured to indicate liver function levels, and the data are expressed as the means ± SEMs (n = 3/group). ^aP < 0.05, ^bP < 0.01, ^cP < 0.001, ^dP < 0.0001. NS represents not statistically significant. All P values were obtained by one-way ANOVA. MenSCs: Menstrual blood-derived stromal cells; IFN-γ: Interferon-γ; IR: Ischemia-reperfusion; IRM: Combination treated with ischemia-reperfusion and menstrual blood-derived stromal cells; IRM (IFN-γ): Combination treated with ischemia-reperfusion and interferon-γ-primed menstrual blood-derived stromal cells; HE: Hematoxylin and eosin; TUNEL: Terminal deoxynucleotidyl transferase-mediated dUTP-biotin Nick End Labeling; ALT: Alanine aminotransferase; AST: Aspartate aminotransferase.